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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in many tumors, which ends up in chromosome instability, and thus may well contribute to tumorigenesis. This protein was found to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant position in advertising mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have already been explained. Two supplemental splice variants were being noted but specific nucleotide sequence facts is not but readily available.
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Blocking peptides are peptides that bind particularly towards the concentrate on antibody and block antibody binding. These peptide ordinarily contains the epitope regarded via the antibody.
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This guideline outlines further more doable optimization of cellular and immunoassay parameters to ensure the absolute best final results are...
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This guideline outlines more probable optimization of cellular and immunoassay parameters to be sure the very best success are...
Antibodies bound to the blocking peptide not bind towards the epitope about the focus on protein. By evaluating the staining from the blocked antibody versus the antibody alone, one can see which staining is particular.
Goal Details Cyclin E belongs on the remarkably conserved cyclin spouse and children, whose members are characterised by a spectacular periodicity in protein abundance from the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation styles which lead towards the temporal coordination of each mitotic event. Cyclin E sorts a fancy with and functions as a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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